FIGURE

Fig. 10

ID

ZDB-FIG-131009-30

Publication

Drummond et al., 2013 - The role of Zic transcription factors in regulating hindbrain retinoic acid signaling

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Fig. 10

Vagal neuron domain in Zic2a and Zic2b morpholino injected embryos is partially rescued with low doses of RA. Dorsal views of the vagal motor neurons in 48 hpf Tg(isl1:eGFP) embryos that were injected with p53 morpholino (A-C) or Zic2a;Zic2b and p53 morpholinos (D-F), and treated with 1% DMSO (A, D), 1 nM RA (B, E), or 1 μM DEAB (C, F). Quantification of vagal motor neuron domain area (G) demonstrates that Zic2a/2b depletion causes a statistically significant reduction in domain size, which is rescued by treatment with retinoic acid. Quantification of vagal neuron domain length (H) gives similar results, with a statistically significant reduction upon Zic2 depletion, which could again be counteracted by retinoic acid addition. Statistical significance calculated by ANOVA and Tukey’s HSD post-hoc test (*p-value d 0.01).

Expression Data

Gene:	GFP
Fish:	rw0Tg rw0Tg + MO2-zic2b + MO3-zic2a + MO4-tp53
Conditions:	chemical treatment: all-trans-retinoic acid chemical treatment: 4-(diethylamino)benzaldehyde
Knockdown Reagents:	MO2-zic2b MO3-zic2a MO4-tp53
Anatomical Term:	motor nucleus of vagal nerve
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rw0Tg + MO2-zic2b + MO3-zic2a + MO4-tp53
Knockdown Reagents:	MO2-zic2b MO3-zic2a MO4-tp53
Observed In:	motor nucleus of vagal nerve anatomical region
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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