Fig. 7

MO knockdown of pes phenocopies Sbds^ATG MO-induced p53-independent defects in pancreas development. (A) Injection of Pes^ATG MO into p53^zdf1/zdf1 embryos results in a dose-dependent defect in pancreatic progenitor expansion, as assessed in 72-hpf ptf1a:eGFP;ins:mCherry embryos. (B) Quantification of three independent experiments scored for the percentage of embryos with wild-type pancreas following injection of Pes^ATG MO into a p53^zdf1/zdf1;ptf1a:eGFP^jh1;ins:mCherry^jh2 background. (C) The gross appearance of Pes^ATG MO-injected embryos at 72 hpf includes a small head and eye but normal body axis. (D) Microarray quantification of pes transcript levels indicates an increase in pes expression in Sbds^ATG MO-injected p53^zdf1/zdf1 embryos. (E) Increased abundance of pes transcripts in Sbds^ATG MO-injected p53^zdf1/zdf1 embryos is confirmed by in situ hybridization. Error bars indicate s.e.m.