FIGURE

Fig. S2

ID

ZDB-FIG-120816-3

Publication

Provost et al., 2012 - Ribosomal biogenesis genes play an essential and p53-independent role in zebrafish pancreas development

Other Figures

All Figure Page

Back to All Figure Page

Fig. S2

Further characterization of the hematopoietic defects in Sbds^ATG MO-injected embryos. (A) Immunofluorescence for L-plastin identifies macrophages at 24 hpf in both Sbds^ATG MO-injected and control embryos. (B) Normal development of vasculature in both Sbds^ATG MO-injected and control embryos as demonstrated by Tg(kdrl:GRCFP)^zn1 transgene. (C) In situ hybridization for gata1, a marker of erythroid progenitors, is identical in control and Sbds^ATG MO-injected embryos at 24 hpf. (D) Epithelial injury does not recruit neutrophils in Sbds^ATG MO-injected embryos. Tails were wounded (arrow) at 24 hpf, and neutrophils were visualized at 30 hpf by in situ hybridization for mpo. (E) In situ hybridization for spi1, a marker of common neutrophil/macrophage progenitors, is identical in control and Sbds^ATG-MO embryos at 24 hpf (arrows). (F) Expression of Tg 9.0 spi1:eGFP at 24 hpf and 48 hpf shows no change in the number or localization of neutrophil/macrophage progenitor markers in Sbds^ATG MO-injected embryos. Note that expression of eGFP in the muscle is not related to hematopoietic development.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development