FIGURE

Fig. 1

ID

ZDB-FIG-120816-6

Publication

Provost et al., 2012 - Ribosomal biogenesis genes play an essential and p53-independent role in zebrafish pancreas development

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Fig. 1

Zebrafish sbds is an essential developmental gene with high-level expression in pancreas and surrounding tissues. (A) Expression of sbds examined by in situ hybridization in wild-type zebrafish embryos at 120 hpf. The boxed pancreatic region is shown at higher magnification to illustrate pancreatic and peri-pancreatic expression (arrowheads) of sbds. (B) Adult zebrafish pancreas (boxed) expresses sbds. (C) Sbds^Spl MO results in durable loss of sbds transcript as assessed by RT-PCR. (D) Survival curve for recipients of Sbds^ATG MO illustrates near complete embryonic lethality by 7 dpf, as compared with mock injected embryos. (E) Ribosome profile of lysate prepared from 48-hpf control and Sbds^ATG MO-injected embryos. (F) Quantification of ribosomal subunit ratios, monosomes and polysomes in control and Sbds^ATG MO-injected embryos. Error bars indicate mean ± s.e.m. i, intestine; L, liver; P, pancreas.

Expression Data

Gene:	sbds
Fish:	WT
Anatomical Term:	pancreas
Stage Range:	Day 5 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO4-sbds
Knockdown Reagent:	MO4-sbds
Observed In:	whole organism whole organism cytosolic ribosome assembly whole organism cytosolic small ribosomal subunit
Stage Range:	Long-pec to Days 7-13

Phenotype Detail

Acknowledgments

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