FIGURE

Fig. 5

ID

ZDB-FIG-120810-59

Publication

Lee et al., 2012 - Disruption of the folate pathway in zebrafish causes developmental defects

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Fig. 5

Methotrexate–treated embryos maintain expression of appropriate developmental markers. Expression patterns were visualized via whole-mount in situ hybridization in embryos treated with 400 μM MTX and fixed at shield stage (A-H), tailbud (I-P), and 24 hpf (Q-W′). While MTX-treated embryos at 24hpf are clearly delayed with respect to normal development, they do express appropriate developmental markers: krt18 –enveloping layer, gsc – involuting cells and neural plate, ntl – blastomere margin, notochord and dorsal organizer, foxA2 – involuting cells and neural plate, crestin – neural crest, myoD – paraxial mesoderm, hgg- hatching gland (anterior most structure), ngn – CNS. (A-J) Animal view, dorsal to the right. (K-P) Lateral view with dorsal view inset. (Q, R, R′, T, V, W, X, X′) Dorsal view. (S, U, V′) Lateral view. (T′) ventral view.

Expression Data

Genes:	crestin ctslb foxa2 gsc krt18a.1 myod1 neurog1 tbxta
Fish:	AB/TU
Condition:	chemical treatment: methotrexate
Anatomical Terms:	central nervous system EVL hatching gland margin neural crest neural plate notochord paraxial mesoderm periderm shield
Stage Range:	Shield to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.