FIGURE

Fig. 4

ID

ZDB-FIG-120810-58

Publication

Lee et al., 2012 - Disruption of the folate pathway in zebrafish causes developmental defects

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

dhfrmorpholino injection phenocopies methotrexate treatment defects. A) Epiboly in dhfr ATG morphants is significantly delayed at 10 hpf as compared to controls. By 28 hpf severe dhfr ATG morphants are developmentally arrested with a failure to complete epiboly. Intron-exon splice morphants complete epiboly but exhibit shortened anterior-posterior axis and heart defects. Embryos in lateral view. B) Diagram of dhfr mRNA, i3e4 splice blocking morpholino, and primers used for RT-PCR (sequences supplied in Additional file 2: Table S2). cDNA generated from embryos at 6 hpf demonstrate splicing defects in dhfr transcripts in embryos treated with morpholino (mut). Control injected embryos (con) have only expected band sizes.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + MO1-dhfr AB/TU + MO2-dhfr
Knockdown Reagents:	MO1-dhfr MO2-dhfr
Observed In:	embryo development epiboly involved in gastrulation with mouth forming second heart heart looping thigmotaxis whole organism whole organism anterior-posterior axis whole organism ventral region
Stage Range:	Bud to Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.