FIGURE

Fig. 6

ID

ZDB-FIG-120810-60

Publication

Lee et al., 2012 - Disruption of the folate pathway in zebrafish causes developmental defects

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Fig. 6

Abnormal cell cycle properties of methotrexate treated embryos A) After 24 hours of 200 μM or 400 μM methotrexate exposure, embryos are capable of incorporating BrdU via DNA synthesis. B) Immunohistochemistry for the mitotic marker phospho-histone H3 shows a decreased number of mitotic cells in methotrexate treated embryos. C) However, FACS analysis clearly indicates methotrexate treated embryos do not display WT cell cycling profiles, with a probable S-phase accumulation. Co-treatment with folinic acid and methotrexate restores cell cycle profiles. Lateral views of 24 hpf embryos. Scale bar: 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.