FIGURE

Fig. 1

ID

ZDB-FIG-120601-72

Publication

van der Velden et al., 2012 - The Polycomb group protein Ring1b is essential for pectoral fin development

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Fig. 1

Generation of ring1b mutants. (A) Schematic representation of the zebrafish ring1b gene depicting the location of the ZFN target site. (B) Wild-type ring1b sequence is shown at the top; the ZFN target sites are highlighted in yellow. ZFN-induced bp insertions are highlighted in red, and deletions in gray. (C,C′) ring1b mRNA staining in brain and pectoral fins in wild-type larvae. (D,D′) Expression is absent in ring1b mutants. (E,F) Ring1b (E) and mono-ubiquitylated histone H2A (F) are not detected in 72 hpf ring1b mutants by western blot analysis. (G,H) Dorsal view of wild-type (G) and ring1b (H) larvae at 72 hpf. Asterisks indicate the lack of pectoral fins in ring1b mutants.

Expression Data

Gene:	rnf2
Fish:	WT rnf2^ibl30/ibl30 rnf2^ibl31/ibl31
Anatomical Terms:	brain pectoral fin
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rnf2^ibl30/ibl30 rnf2^ibl31/ibl31
Observed In:	histone H2A ubiquitin ligase activity pectoral fin
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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