FIGURE

Fig. 4

ID

ZDB-FIG-110928-13

Publication

Xie et al., 2011 - Zebrafish foxo3b Negatively Regulates Canonical Wnt Signaling to Affect Early Embryogenesis

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Fig. 4

Knockdown of foxo3b leads to defects in DV patterning during early embryogenesis.

(A) A1–A4, sqt expression increased in foxo3b-MO injected embryos compared to control embryos. A5–A8, presumptive organizer marker flh expanded at foxo3b-knockdown embryos. A9–A10, foxo3b knockdown caused ectopic vox expression at 30% epiboly. (B) The expression of presumptive organizer marker gsc and flh in foxo3b-MO injected embryos. The arrows identified embryos with expanded expression (B2, 74%, n = 23; B4, 52%,n = 21, respectively) and asterisks identified embryos with decreased expression. Embryos were injected with 8 ng STD-MO (control) or 8 ng foxo3b-ATG-MO. A1–A4, lateral views; A5–A12, B1–B4, animal pole views; A1–A12, 30% epiboly; B1–B4, shield stage.

Expression Data

Genes:	gsc ndr1 noto
Fish:	AB WT AB + MO1-foxo3b WT + MO1-foxo3b
Knockdown Reagent:	MO1-foxo3b
Anatomical Terms:	presumptive shield shield unspecified
Stage Range:	30%-epiboly to Shield

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-foxo3b
Knockdown Reagent:	MO1-foxo3b
Observed In:	dorsal/ventral pattern formation
Stage:	30%-epiboly

Phenotype Detail

Acknowledgments

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