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Fig. 4
Knockdown of foxo3b leads to defects in DV patterning during early embryogenesis.
(A) A1–A4, sqt expression increased in foxo3b-MO injected embryos compared to control embryos. A5–A8, presumptive organizer marker flh expanded at foxo3b-knockdown embryos. A9–A10, foxo3b knockdown caused ectopic vox expression at 30% epiboly. (B) The expression of presumptive organizer marker gsc and flh in foxo3b-MO injected embryos. The arrows identified embryos with expanded expression (B2, 74%, n = 23; B4, 52%,n = 21, respectively) and asterisks identified embryos with decreased expression. Embryos were injected with 8 ng STD-MO (control) or 8 ng foxo3b-ATG-MO. A1–A4, lateral views; A5–A12, B1–B4, animal pole views; A1–A12, 30% epiboly; B1–B4, shield stage.