FIGURE

Fig. 6

ID

ZDB-FIG-110928-15

Publication

Xie et al., 2011 - Zebrafish foxo3b Negatively Regulates Canonical Wnt Signaling to Affect Early Embryogenesis

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Fig. 6

Foxo3b functions in posterior neuroectoderm formation and involves with BMP signaling in DV patterning.

(A) Foxo3b affected posterior neuroectoderm formation and mesoderm induction. A1-A2, at 80% epiboly, cdx4 expression expanded in foxo3b-MO injected embryos compared to control embryos. A3-A4, the expression of non-neural ectodermal marker gata2 was up-regulated in foxo3b-knockdown embryos. A5-A6, ectoderm marker foxi1 expanded in foxo3b morphants. (B) The expression of wnt8 and bmp ligands in foxo3b morphants. B1–B2, wnt8 expression increased in foxo3b morphants at shield stage. B3–B6, the expression of ventral markers bmp2b/bmp4 was up-regulated in foxo3b-knockdown embryos at shield stage. Embryos were injected with 8 ng STD-MO (control) or 8 ng foxo3b-ATG-MO. A1–A2, lateral views with dorsal to the right; A3–A6, animal pole views with dorsal to the right; B1–B6, lateral views with dorsal to the right; A1–A2, 80% epiboly; A3–A6, B1–B6, shield stage.

Expression Data

Genes:	bmp2b bmp4 cdx4 foxi1 gata2a wnt8a
Fish:	WT WT + MO1-foxo3b
Knockdown Reagent:	MO1-foxo3b
Anatomical Term:	epiblast
Stage:	Shield

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-foxo3b
Knockdown Reagent:	MO1-foxo3b
Observed In:	dorsal/ventral pattern formation neural tube patterning Wnt signaling pathway
Stage:	Shield

Phenotype Detail

Acknowledgments

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