FIGURE

Fig. 6

ID

ZDB-FIG-110920-74

Publication

Eames et al., 2011 - Mutations in fam20b and xylt1 Reveal That Cartilage Matrix Controls Timing of Endochondral Ossification by Inhibiting Chondrocyte Maturation

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Fig. 6

fam20b mutants display reduced proteoglycan immunoreactivity

A–F, Ventral views of confocal images of embryonic heads immunostained for chondroitin sulfate proteoglycan (CSPG) at 3 dpf (A,B) or heparan sulfate PG (HSPG) at 4 dpf (C–F). Compared to abundant CSPGs detected in wild-type skeletal elements (A), fam20b^b1127 mutants showed decreased CSPG immunoreactivity (B). HSPGs were abundant in gill filaments and ventral muscle groups of both wild-type (C) and fam20b^b1127 mutant zebrafish (D). HSPG immunoreactivity was reduced greatly around developing chondrocytes of the fam20b^b1127 mutant ceratohyal (outline in F), compared to those of wild-type siblings (outline in E). Also, the number of intracellular HSPG aggregates (arrowhead) was increased in fam20b^b1127 mutant chondrocytes. Abbreviations: A = anterior; αCS = anti-chondroitin sulfate; αHS = anti-heparan sulfate; c = chondrocyte; ch = ceratohyal; e = eye; gf = gill filament; ih = interhyoideus; imp = intermandibularis posterior; Mk = Meckel′s; op = opercle; P = posterior; pq = palatoquadrate.

Expression Data

Antibodies:	Ab-10E4 Ab-CS-56
Fish:	WT fam20b^b1127/b1127 (AB)
Anatomical Terms:	ceratohyal cartilage gill filament interhyoideus Meckel's cartilage opercle palatoquadrate cartilage ventral intermandibularis posterior
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fam20b^b1127/b1127 (AB)
Observed In:	ceratohyal cartilage chondrocyte proteoglycan biosynthetic process
Stage:	Day 4

Phenotype Detail

Acknowledgments

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