FIGURE

Fig. 10

ID

ZDB-FIG-110920-78

Publication

Eames et al., 2011 - Mutations in fam20b and xylt1 Reveal That Cartilage Matrix Controls Timing of Endochondral Ossification by Inhibiting Chondrocyte Maturation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 10

Early molecular markers of chondrocyte maturation in fam20b and xylt1 mutants.

A–R, horizontal section in situ hybridization of developing ceratohyals. The levels of runx2b transcripts appeared higher in chondrocytes (c) and perichondrium (pc) of the ceratohyal in fam20b^b1127 (B) and xylt1^b1128 (C) mutants, than in wild types (A) at 72 hpf. Expression of col10a1 was abundant in chondrocytes (c) and perichondrium (pc) of the ceratohyal in fam20b^b1127 (E) and xylt1^b1128 (F) mutants, but was not detectable in wild types (D) at 83 hpf. Levels of ihha and ihhb transcripts were up-regulated in chondrocytes (c) of the ceratohyal in fam20b^b1127 (H,K) and xylt1^b1128 (I,L) mutants, but were not detectable in wild types (G,J) at 72 hpf. Transcript levels for ptch2 were increased in the perichondrium of fam20b^b1127 (N) and xylt1^b1128 (O) mutants, compared to wild types (M) at 72 hpf, whereas no obvious differences in levels of ptch1 expression in perichondria were apparent (P–R). Abbreviations: c = chondrocytes; pc = perichondrium.

Expression Data

Genes:	col10a1a ihha ihhb ptch1 ptch2 runx2b
Fish:	AB fam20b^b1127/b1127 (AB) xylt1^b1128/b1128 (AB)
Anatomical Terms:	ceratohyal cartilage chondrocyte ceratohyal cartilage perichondrium
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fam20b^b1127/b1127 (AB) xylt1^b1128/b1128 (AB)
Observed In:	chondrocyte development perichondral bone
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.