FIGURE

Fig. 11

ID

ZDB-FIG-110920-79

Publication

Eames et al., 2011 - Mutations in fam20b and xylt1 Reveal That Cartilage Matrix Controls Timing of Endochondral Ossification by Inhibiting Chondrocyte Maturation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 11

Ultrastructural evidence of premature chondrocyte hypertrophy and aberrant matrix production in xylt1 mutants.

A–D, transmission electron micrographs of 84 hpf ceratohyals. At 5600X magnification, chondrocytes in the central region of the wild-type ceratohyal displayed abundant rough ER, Golgi, and mitochondria in the cytoplasm (A). Similar views of xylt1 mutant chondrocytes at the same age and comparable positions within the developing ceratohyal showed cytoplasmic clearing and tremendous reduction in biosynthetic organelles (B). Mutant chondrocytes also appeared to be separated by less extracellular matrix. While wild-type (C) and mutant (D) extracellular matrix demonstrated fibrillar collagens at 31000X magnification, mutant matrix showed tighter fibril packing and also contained more amorphous electron-dense structures (*) in pericellular matrix than seen in wild types. Abbreviations: cyt = cytoplasm; ecm = extracellular matrix; nuc = nucleus. Scale bars: A,B = 2 μm; C,D = 500 nm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	xylt1^b1128/b1128 (AB)
Observed In:	chondrocyte hypertrophy hypertrophic chondrocyte
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.