ZFIN Image: Eames et al., 2011, Fig. 6

Image

Figure Caption

Fig. 6

fam20b mutants display reduced proteoglycan immunoreactivity

A–F, Ventral views of confocal images of embryonic heads immunostained for chondroitin sulfate proteoglycan (CSPG) at 3 dpf (A,B) or heparan sulfate PG (HSPG) at 4 dpf (C–F). Compared to abundant CSPGs detected in wild-type skeletal elements (A), fam20b^b1127 mutants showed decreased CSPG immunoreactivity (B). HSPGs were abundant in gill filaments and ventral muscle groups of both wild-type (C) and fam20b^b1127 mutant zebrafish (D). HSPG immunoreactivity was reduced greatly around developing chondrocytes of the fam20b^b1127 mutant ceratohyal (outline in F), compared to those of wild-type siblings (outline in E). Also, the number of intracellular HSPG aggregates (arrowhead) was increased in fam20b^b1127 mutant chondrocytes. Abbreviations: A = anterior; αCS = anti-chondroitin sulfate; αHS = anti-heparan sulfate; c = chondrocyte; ch = ceratohyal; e = eye; gf = gill filament; ih = interhyoideus; imp = intermandibularis posterior; Mk = Meckel′s; op = opercle; P = posterior; pq = palatoquadrate.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.