FIGURE

Fig. 1

ID

ZDB-FIG-090320-71

Publication

Jing et al., 2009 - Wnt signals organize synaptic prepattern and axon guidance through the zebrafish unplugged/MuSK receptor

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Fig. 1

UnpSV1 Controls AChR Prepatterning

(A) Domain structure of the Unplugged protein isoforms.

(B–E) Lateral views of caudal segments in 17 hpf embryos stained for motor axons (green, znp-1/SV2) and AChRs (red, α-BTX). (B) In wild-type embryos, AChRs are prepatterned in a central band along the dorsal and ventral myotome before the first growth cones approach. (C) UnpFL MO injection does not affect AChR prepattern. (D) UnpSV1 MO injection causes complete absence of AChR prepattern. (E) UnpSV1 expression in adaxial cells restores AChR prepattern in unplugged mutant embryos.

(F and G) Lateral views and cross-sectional views (H and I) of 17 hpf Tg(smyhc1:UnpSV1myc) embryos stained with anti-myc (green) and anti-Prox1 (red), which labels the nuclei of adaxial cells.

Scale bars, 50 μm.

Expression Data

Genes:	chrna1 prox1a
Antibodies:	Ab1-myc znp-1
Fish:	WT WT + MO1-musk WT + MO2-musk p147Tg musk^{tbr307/tbr307}; p147Tg
Knockdown Reagents:	MO1-musk MO2-musk
Anatomical Terms:	adaxial cell adaxial cell nucleus motor neuron axon myotome acetylcholine-gated channel complex myotome plasma membrane
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-musk
Knockdown Reagent:	MO1-musk
Observed In:	skeletal muscle acetylcholine-gated channel clustering
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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