FIGURE

Fig. 5

ID

ZDB-FIG-090320-75

Publication

Jing et al., 2009 - Wnt signals organize synaptic prepattern and axon guidance through the zebrafish unplugged/MuSK receptor

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Inhibition of the Noncanonical Dsh Pathway in Adaxial Fibers

(A) Stochastic expression of Tg(smyhc1:GFP) in adaxial muscle (green) does not affect motor axons (red).

(B) Expression of Tg(smyhc1:myc-XDsh-DEP+) (green) in adaxial fibers dorsal to the choice point causes unplugged-like pathfinding defects (arrow).

(D) Analysis of axonal phenotypes. n, hemisegments; blue, hemisegments with two adaxial cells expressing the transgene; yellow, hemisegments with three or more adaxial cells expressing the transgene.

(E–F′) Confocal images of adjacent adaxial muscle pioneers expressing the smyhc1-GFP or smyhc1-myc-Xdsh-DEP+ transgene. Only AChR clusters between two adjacent transgene-positive adaxial cells were analyzed (outlined by dashed lines). Tg(smyhc1:GFP) expressing adaxial cells form AChR clusters (arrowheads in [E′]), while Tg(smyhc1:myc-XDsh-DEP+ expression disrupts AChR clusters between transgene expressing cells ([F′], open arrowhead); note that this does not affect adjacent, nontransgenic cells that formed normal AChR clusters ([F′], arrows). For each transgene, four embryos with GFP or Myc-Dsh-DEP+ positive adaxial cells were analyzed. Prepatterned clusters were reduced in all Myc-Dsh-DEP+ expressing embryos.

Scale bars: (A) 50 μm, (E) 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neuron