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Fig. 1 UnpSV1 Controls AChR Prepatterning
(A) Domain structure of the Unplugged protein isoforms.
(B–E) Lateral views of caudal segments in 17 hpf embryos stained for motor axons (green, znp-1/SV2) and AChRs (red, α-BTX). (B) In wild-type embryos, AChRs are prepatterned in a central band along the dorsal and ventral myotome before the first growth cones approach. (C) UnpFL MO injection does not affect AChR prepattern. (D) UnpSV1 MO injection causes complete absence of AChR prepattern. (E) UnpSV1 expression in adaxial cells restores AChR prepattern in unplugged mutant embryos.
(F and G) Lateral views and cross-sectional views (H and I) of 17 hpf Tg(smyhc1:UnpSV1myc) embryos stained with anti-myc (green) and anti-Prox1 (red), which labels the nuclei of adaxial cells.
Scale bars, 50 μm.