Fig. S3

Genetic interactions between unplugged and wnt11r.
(A) Quantification of motor axon defects at 27 hpf using suboptimal wnt11r TL-MO dose (2 ng). unplugged/+ embryos are indistinguishable from wildtype embryos. The same amount of sub-optimal dose of wnt11r TL-MO injected into wildtype embryos resulted in 13% unplugged-like axonal defects, while injection into unplugged/+ embryos increased the phenotype to 23%. Twenty hemisegments were scored in each embryo; n=hemisegments. Results are compiled from multiple experiments as the average±S.E.M. (t-test, *p<0.01). (B-C) Analysis of prepatterning phenotype using optimal wnt11r TL MO concentrations (3-4 ng) . (B1-B3) Each hemisegment of embryos was scored as normal prepatterning, mild reduction or strong reduction. (C) Quantification data show prepatterning phenotype was increased in unplugged/+ embryos. Results were obtained from three different experiments; n= hemisegments. 3 or 4 hemisegments were scored in each embryo.