FIGURE

Fig. 2

ID

ZDB-FIG-080604-24

Publication

Laue et al., 2008 - The multidomain protein Brpf1 binds histones and is required for Hox gene expression and segmental identity

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Fig. 2

Brpf1 regulates segmental identity by maintaining anterior Hox gene expression. Whole-mount in situ hybridizations with the probes indicated bottom left at the stages indicated bottom right; genotypes and treatment of zebrafish embryos as indicated in upper right corners. (A-L) Lateral views; (M-P) ventral views. (A-D) Hox gene expression in wild type (WT). Hox-expressing hindbrain rhombomeres (r) and arch-forming cranial neural crest (CNC) (2-7) are indicated. sc, spinal cord. (E-H) Absent or reduced Hox gene expression in brpf1 mutants (-/-). Arrow in H indicates the remaining hoxb3a expression in the posterior CNC. (I-L) Partially rescued Hox gene expression in the hindbrain (I, arrow) and the CNC (J-L, arrows) of brpf1 mutants injected with mouse Brpf1 mRNA. (M-P) The bimandibular phenotype of the brpf1 mutant (N) can be overcome by injection of hoxb1a mRNA. (O) hoxb1a-injected wild-type embryo lacking bapx1 expression, indicative for bihyoid phenotype [compare with Hunter and Prince (Hunter and Prince, 2002)]. (P) hoxb1a-injected brpf1 mutant with bihyoid pattern on left side and wild-type pattern on right side. Arch numbers are indicated.

Expression Data

Genes:	hoxa2b hoxb1a hoxb2a hoxb3a nkx3-2
Fish:	WT brpf1^{t20002/t20002}
Anatomical Terms:	pharyngeal arch 1 pharyngeal arch 2 pharyngeal arch 3-7 rhombomere 2 rhombomere 3 rhombomere 4 rhombomere 5 spinal cord
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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