Control experiments. Dorsal views (A-O). (A,B) Embryos double stained with myoD (red) and vasa (blue), 14 h. vasa riboprobe ensures that ody-/- was correctly identified since mutant embryos appear phenotypically normal. No significant change in myoD was observed in ody-/-. Arrows indicate the cluster of primodial germ cells (PGCs). (C,D) Embryos double stained with myf5 (blue) and vasa (red), 14 h. No significant change was detected in the paraxial mesoderm. Insets showing normal cluster of PGCs in ody sib and PGCs were found along midline in head region of ody-/-. (E-J) Two sets of experiment (E-G and H-J) demonstrating redundancy in function between cxcr4a and cxcr4b. A lower dosage of cxcr4a MO was used to obtain normal myoD staining but disrupted myoD in paraxial mesoderm in ody -/-. vasa (red) helps to identify ody -/-. White arrowheads in J indicate ectopic expression of myoD. (K-M) cxcr4a and sdf1a morphants show normal transcription of myf5 in tailbud domain, 14 h. (N,O) A representative e12 morphant stained with deltaC as a control for cxcr4a and sdf1a transcriptional analysis, 14 hpf. Somites are formed in these morphants and notch pathway is unaffected.
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