FIGURE

Fig. S4

ID

ZDB-FIG-071015-7

Publication

Chong et al., 2007 - The chemokine, Sdf-1, and its receptor, Cxcr4, are required for formation of muscle in zebrafish

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Fig. S4

Efficiency of splice site MOs. (A,C,G,K) β-actin primers used in +RT control. Splice site MOs inhibit splicing in sdf1a (B), myoD (D), myf5 with degradation (H) and e12 (L). Total RNA from control (lane1,5), 0.46 pmole/embryo (lane2,6), 0.92 pmole/embryo (lane 3,7), 2.3 pmole/embryo(lane4,8). Splice product sizes are indicated by white asterisks. (E,F) mylz2 riboprobe staining on control (n = 20/20) and myoD-EI morphant (n = 19/20) respectively [96]. (I,J) myogenin riboprobe staining on control (n = 20/20) and myf5-EI morphant (n = 18/20) respectively [97]. (M,N) sdf1a riboprobe staining on E12-EI morphant (n = 20/20)and morphant rescued with e12 mRNA (n = 10/16).

Expression Data

Gene:	myog
Fish:	WT WT + MO4-myf5
Knockdown Reagent:	MO4-myf5
Anatomical Term:	somite
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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