FIGURE

Fig. 3

ID
ZDB-FIG-070810-13
Publication
Chong et al., 2007 - The chemokine, Sdf-1, and its receptor, Cxcr4, are required for formation of muscle in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 3

Formation of fast muscle requires Cxcr4a and Sdf1a. Lateral views (A-F), cross-section (G,H), sagittal section (I,J) and dorsal views. (A) Birefringence revealed by polarized light in cxcr4a (II) and sdf1a (III) morphants was reduced compared to control (I), 30 h. (B) Schematic illustrating black box region used for imaging. Start of yolk sac extension as a guide for the center of frame, indicated by dashed line in diagram of zebrafish embryo. (C-F) Single confocal images taken at level of the somite boundary as a guide of depth. Myosin light chain transgenic line, 51 h. (C,D) mcxcr4a (n = 87/87) and msdf1a (n = 31/35) morphants developed normally. (E,F) In representative cxcr4a (n = 63/71) and sdf1a (n = 73/82) morphants, reduction of GFP signal was observed. (G-J) Transmission electron micrograph of cross (G,H) and sagittal (I,J) sections in trunk region of control and cxcr4a morphants respectively, 36 h. A representative cxcr4a morphant clearly shows a reduction in muscle fibrils. (I,J) Black arrows indicate lack or absence of sarcoplasmic reticulum and muscle fibers in some areas of cxcr4a morphant. For clarity, this region of section (J) was selected where there are at least some muscle fibers. Abbreviations: HM – horizontal myoseptum; ys – yolk sac and yse – yolk sac extension. Scale bars = 500 nm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.