FIGURE

Fig. S2

ID

ZDB-FIG-061219-9

Publication

Mendelsohn et al., 2006 - Atp7a determines a hierarchy of copper metabolism essential for notochord development

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Fig. S2

atp7a morpholino causes abnormal splicing of the zebrafish atp7a transcript A) PCR amplification from cDNA of the region surrounding the target of this MO reveals a single band of expected size from embryos injected with control MO, and an additional larger band (arrowhead) in embryos injected with MO to atp7a. B) Sequence analysis of this band reveals a transcript resulting from a cryptic splice donor 44 nt into the intron, causing a frame shift. The predicted resulting ORF, when translated, would contain 46 incorrect amino acids and terminate prematurely, removing the final 354 amino acids, including two transmembrane domains.

Expression Data

Gene:	atp7a
Fish:	WT + MO1-atp7a
Knockdown Reagent:	MO1-atp7a
Anatomical Term:	whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell Metabolism, 4(2), Mendelsohn, B.A., Yin, C., Johnson, S.L., Wilm, T.P., Solnica-Krezel, L., and Gitlin, J.D., Atp7a determines a hierarchy of copper metabolism essential for notochord development, 155-162, Copyright (2006) with permission from Elsevier. Full text @ Cell Metab.