ZFIN Image: Mendelsohn et al., 2006, Fig. S2

Image

Figure Caption

Fig. S2 atp7a morpholino causes abnormal splicing of the zebrafish atp7a transcript A) PCR amplification from cDNA of the region surrounding the target of this MO reveals a single band of expected size from embryos injected with control MO, and an additional larger band (arrowhead) in embryos injected with MO to atp7a. B) Sequence analysis of this band reveals a transcript resulting from a cryptic splice donor 44 nt into the intron, causing a frame shift. The predicted resulting ORF, when translated, would contain 46 incorrect amino acids and terminate prematurely, removing the final 354 amino acids, including two transmembrane domains.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell Metabolism, 4(2), Mendelsohn, B.A., Yin, C., Johnson, S.L., Wilm, T.P., Solnica-Krezel, L., and Gitlin, J.D., Atp7a determines a hierarchy of copper metabolism essential for notochord development, 155-162, Copyright (2006) with permission from Elsevier. Full text @ Cell Metab.