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Fig. 3

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ZDB-FIG-061219-3

Publication

Mendelsohn et al., 2006 - Atp7a determines a hierarchy of copper metabolism essential for notochord development

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Fig. 3

Small-molecule effects are rapid, saturable, and dose and time dependent

Examination of embryo at 48 hpf following no treatment reveals normal melanin pigmentation and notochord (A), while treatment with 10 μM neocuproine for 10 min at 3 hpf abrogates melanin pigmentation but does not affect the notochord (arrows) (B). Treatment with 10 μM neocuproine for 20 min starting at 3 hpf reveals both abrogated pigmentation, enlarged hindbrain ventricle (arrowhead), and a wavy notochord (arrows) (C). Treatment with 10 μM neocuproine for 60 min at 3 hpf does not increase the severity of the pigment, notochord (arrows), or ventricle (arrowheads) phenotype (D), indicating that the effect is saturable. Untreated 48 hpf embryo (E) shows the normal distribution of melanocytes with melanin pigmentation (inset, arrowhead) and normal notochord, while treatment at 3 hpf with 500 nM neocuproine (one-twentieth of the dose of neocuproine resulting in the complete phenotype) (F) impairs only melanin pigmentation (inset, arrowhead).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell Metabolism, 4(2), Mendelsohn, B.A., Yin, C., Johnson, S.L., Wilm, T.P., Solnica-Krezel, L., and Gitlin, J.D., Atp7a determines a hierarchy of copper metabolism essential for notochord development, 155-162, Copyright (2006) with permission from Elsevier. Full text @ Cell Metab.