- Title
-
ALS-associated genes display CNS expression in the developing zebrafish
- Authors
- Laboissonniere, L.A., Smith, C.L., Mesenbrink, J., Chowdhury, R., Burney, A., Lang, M., Sierra, M., Stark, A., Maldonado-Casalduc, G., Muller, M., Trimarchi, J.M.
- Source
- Full text @ Gene Expr. Patterns
ALS-linked genes at 2dpf in the zebrafish. The expression pattern of 18 ALS-associated genes were investigated using whole mount in situ hybridization in the zebrafish at 2dpf. The genes examined were: (A) prph, (B) matr3l1.2, (C) erbb4a, (D) fig4a, (E) mapta, (F) dctn1b, (G) tia1, (H) vcp, (I) ewsr1a, (J) ewsr1b, (K) hnrnpa1a, (L) hnrnpa1b, (M) chmp2ba, (N) chmp2bb, (O) erbb4b, (P) spg11, (R) fus, and (S) pfn1. Scale bars represent 200 μm. |
Head-only expressed genes in the developing zebrafish. Brain expression of genes that were robustly detected in the head of the developing zebrafish by whole mount in situ hybridization are shown at 2dpf, 3dpf, and 4dpf. Genes belonging to the head-only group were (A-a’’) prph, (B-b’’) matr3l1.2, (C-a’’) erbb4a, (D-d’’) fig4a, (E-e’’) mapta, and (F-f’’) dctn1b. Scale bars represent 200 μm. |
Head and faint trunk expressed genes in the head of the developing zebrafish. Brain expression of genes that were detected in the head and faintly in the trunk of the developing zebrafish by whole mount in situ hybridization are shown at 2dpf, 3dpf, and 4dpf. (A-a’’) tia1, (B-b’’) vcp, (C-c’’) ewsr1a, (D-d’’) ewsr1b, (E-e’’) hnrnpa1a, (F-f’’) hnrnpa1b, and (G-g’’) chmp2ba belonged to this group. Scale bars represent 200 μm. |
Head and robust trunk expressed genes in the head of the developing zebrafish. Brain expression of genes that were grouped in the head and robust trunk expression category are shown at 2dpf, 3dpf, and 4dpf. This final group of genes included (A-a’’) chmp2bb, (B-b’’) erbb4b, (C-c’’) spg11, (D-d’’) fus, and (E-e’’) pfn1. Scale bars represent 200 μm. |
Expression of ALS-linked genes in the trunk of zebrafish during development. The trunks of zebrafish were examined at 4dpf for 18 different ALS-associated genes. (A) prph, (B) matr3l1.2, (C) erbb4a, (D) fig4a, (E) mapta, (F) dctn1b, (G) tia1, (H) vcp, (I) ewsr1a, (J) ewsr1b, (K) hnrnpa1a, (L) hnrnpa1b, (M) chmp2ba, (N) chmp2bb, (O) erbb4b, (P) spg11, (Q) fus, and (R) pfn1. Scale bars represent 200 μm. |
Retinal expression of ALS-linked genes during development (group 1). The retinas of zebrafish were examined at 2dpf, 3dpf, and 4dpf for genes belonging to the head-only expression group. (A-A″) prph, (B-B″) matr3l1.2, (C-C″) erbb4a, (D-D″) fig4a, (E-E″) mapta, and (F-F″) dctn1b. Scale bars represent 100 μm. |
Retinal expression of ALS-linked genes during development (group 2). The retinas of zebrafish were examined at 2dpf, 3dpf, and 4dpf for genes belonging to the head and faint tail expression group. (A-A″) tia1, (B-B″) vcp, (C-C″) ewsr1a, (D-D″) ewsr1b, (E-E″) hnrnpa1a, (F-F″) hnrnpa1b, and (G-G″) chmp2ba. Scale bars represent 100 μm. |
Retinal expression of ALS-linked genes during development (group 3). The retinas of zebrafish were examined at 2dpf, 3dpf, and 4dpf for genes belonging to the head and robust tail expression group. (A-A″) chmp2bb, (B-B″) erbb4b, (C-C″) spg11, (D-D″) fus, and (E-E″) pfn1. Scale bars represent 100 μm. |
ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. |
|
ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. |
|
ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. |
|
ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. EXPRESSION / LABELING:
|
|
ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. EXPRESSION / LABELING:
|
|
Unillustrated author statements |
Reprinted from Gene expression patterns : GEP, 30, Laboissonniere, L.A., Smith, C.L., Mesenbrink, J., Chowdhury, R., Burney, A., Lang, M., Sierra, M., Stark, A., Maldonado-Casalduc, G., Muller, M., Trimarchi, J.M., ALS-associated genes display CNS expression in the developing zebrafish, 14-31, Copyright (2018) with permission from Elsevier. Full text @ Gene Expr. Patterns