FIGURE

Fig. 4

ID

ZDB-FIG-240524-22

Publication

Davison et al., 2024 - Tagging the tjp1a Gene in Zebrafish with Monomeric Red Fluorescent Protein Using Biotin Homology Arms

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Fig. 4

tjp1a-mRFP embryos show localization of mRFP to regions of cell–cell contact. Confocal images of mRFP (b, e, h, k), eGFP from fli1-egfpy1 (a, d, g, j), and merged (c, f, I, l) channels. (a–f) 1 dpf tjp1a-mRFPis86; fli1-egfpy1 embryos imaged with a 5x (a–c) and 20x (d–f) objective. (g–l) 3 dpf tjp1a-mRFP is86; fli1-egfpy1 embryos imaged with a 10x (g–i) and 20x (j–l) objective. All 20 × images are centered around the urogenital opening. eGFP from fli1-egfpn1 is expressed in blood vessels and mRFP from tjp1-mRFP is86 is likely present in tight junctions. mRFP was observed in blood vessels, the central nervous system, and the gut.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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