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Fig. 4 tjp1a-mRFP embryos show localization of mRFP to regions of cell–cell contact. Confocal images of mRFP (b, e, h, k), eGFP from fli1-egfpy1 (a, d, g, j), and merged (c, f, I, l) channels. (a–f) 1 dpf tjp1a-mRFPis86; fli1-egfpy1 embryos imaged with a 5x (a–c) and 20x (d–f) objective. (g–l) 3 dpf tjp1a-mRFP is86; fli1-egfpy1 embryos imaged with a 10x (g–i) and 20x (j–l) objective. All 20 × images are centered around the urogenital opening. eGFP from fli1-egfpn1 is expressed in blood vessels and mRFP from tjp1-mRFP is86 is likely present in tight junctions. mRFP was observed in blood vessels, the central nervous system, and the gut.