FIGURE

Fig. 1

ID

ZDB-FIG-240524-19

Publication

Davison et al., 2024 - Tagging the tjp1a Gene in Zebrafish with Monomeric Red Fluorescent Protein Using Biotin Homology Arms

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Fig. 1

Knock-in strategy for generation of Tjp1a-mRFP fusion. The gene schematic contains blue and green boxes to represent the 5′ and 3′ 35 bp homology arms. The red line shows the expected double-strand break site after injection with Cas9 protein and the cr:tracRNA. Shown below is a schematic of the biotin-labeled template containing the homology arms, LGGGSGGSGASSEDV flexible linker and mRFP. The tjp1a-mRFP knock-in allele schematic depicts the expected integration at the 3′ end of the tjp1a coding sequence. Arrows present on the schematic represent the primer binding sites used to generate junction fragments and verify the presence of the precise integration. mRFP, monomeric red fluorescent protein.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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