Expression of genes involved in neurogenesis and Shh signaling in foxg1a mutants. The expression of neurogenesis-related genes (a–f′) and Shh signaling-related genes (g–j′) was examined by WISH in foxg1a mutants at 24 hpf, followed by genotyping. Wild-type embryos including heterozygotes (left) and homozygotes (right) are shown for each gene. Wild-type and heterozygous embryos showed indistinguishable expression patterns. Black arrowheads indicate the telencephalic ventricular zone. The numbers on the lower right indicate the numbers of embryos with the indicated expression patterns and the numbers of total assessed embryos. Asterisks indicate abnormal expression. Lateral views, anterior to the left and dorsal to the top; dorsal views, anterior to the left. et, epithalamus; fp, floor plate; ht, hypothalamus; poa, preoptic area; re, retina; te, telencephalon; tg, tegmentum; zli, zona limitans intrathalamica. Scale bar, 100 μm.
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