FIGURE

Fig. 3

ID

ZDB-FIG-240313-17

Publication

Auman et al., 2023 - Zebrafish smarcc1a mutants reveal requirements for BAF chromatin remodeling complexes in distinguishing the atrioventricular canal from the cardiac chambers

Other Figures

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Fig. 3

Morpholino knockdown of smarcc1a phenocopies smarcc1ask29 mutants. (A, B) Lateral views of live embryos at 52 hpf, anterior to the left, dorsal to the top. Morpholino-injected embryos (MO) exhibit reduced pigmentation, pericardial edema, and abnormal retinal development. (C, D) Pectoral fins (arrowheads) do not develop normally in MO-injected embryos, resulting in small, stunted fin buds at 52 hpf. (E, F) In contrast to the heart of an uninjected wild-type embryo (arrowhead, E), the heart of a MO-injected embryo (arrowhead, F) has dysmorphic chambers aligned in a linear arrangement. Overall, the phenotypes of MO-injected embryos are highly similar, although somewhat less severe, than those of smarcc1ask29 mutant embryos, likely reflecting the limited efficiency of morpholino knockdown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO4-smarcc1a + MO5-smarcc1a
Knockdown Reagents:	MO4-smarcc1a MO5-smarcc1a
Observed In:	atrioventricular canal melanocyte pectoral fin pericardium retina retinal pigmented epithelium
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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