Figure 1

The osteoblasts at 4 dpf reveal two distinct populations based on the GFP fluorescence intensity and differential gene expression. (A) FACS plot showing forward scattering (FSC-A) and GFP fluorescence in singlet, living cells in gates P1 and P2. Cell distribution according to their GFP fluorescence is also shown to illustrate the two subpopulations P1 and P2. Gates were set to exclude the 100-fold larger population of non-fluorescent cells for illustration. (B) Number of DEGs that are up- or down-regulated in the different comparisons. (C) Venn diagram comparing the DEGs in “AllvsP1”, “AllvsP2”, and “P1vsP2”. (D) PCA plot, based on the 500 most variable genes in terms of normalized read counts in all individual samples, showing that the two cell subpopulations P1 and P2 are clearly different from each other, but very different from the whole larvae “All” population at 4 dpf. (E) Selected terms enriched in the DEG lists “AllvsP1”, “AllvsP2”, and “P1vsP2” as determined by GSEA analysis; columns represent the list concerned, the database used, the dataset concerned, its name, the normalized enrichment score, and the false discovery rate value (FDR). Positive enrichment scores indicate up-regulated terms (highlighted in red), negative ones refer to down-regulated terms (highlighted in green).