Figure 1

The ARMS-PCR technique allows to discriminate between WT and mutant alleles. (A) The ARMS-PCR principle is illustrated here. The single point mutation in the elna^sa12235 mutant corresponds to the substitution of thymine into an adenine (delineated in red). Two pairs of primers are used so that PCR amplification does not lead to the same result for WT and mutant alleles. Crosses symbolize a mismatch between the primer and the DNA sequence. When two mismatches are found at the 3′ extremity of the primer, the elongation cannot happen. When only one mismatch is present, transcription can occur (black arrow). (B) Schematic representation of amplicons generated through ARMS-PCR. (C) Gel electrophoresis of the PCR amplification products enables the genotype to be revealed for WT (wild type), HET (heterozygous), or MUT (mutant) individuals.