elnasa12235/+ and elnasa12235/sa12235 mutants suffer from severe cardiac complications. (A) Mutant zebrafish from 9 mpf were found to undergo complications such as (iii) an important swelling of the belly unrelated to stuck eggs; (iv,v) swelling of the entire body and protrusion of the scales with a rapid aggravation; (iv) early stage; (v) late stage; (vi) ulcers on their body; (i,ii) show normal fish of the same age. White arrows point to major observations of each image; scale bar = 0.5 cm. (B) Histological sections from (i,iii) normal or (iv–vi) pathological hearts from sick fish with protruding scales (here from elnasa12235/+ fish) were labeled for (i,iv) elastin; (ii,v) lipids; and (iii,vi) CD3e. In (iv) and (v), dotted lines encircle the dysplastic tissue surrounding the bulbus arteriosus; black arrows point to “nodule-like” structures found in the pathological hearts; scale bar = 200 µm, except for zooms in vi-1 and vi-2, where scale bar = 20 µm. BA = bulbus arteriosus; V = ventricle; A = atrium; mpf = months post fertilization.
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