Figure 5

elna mutants show abnormal cardiac valves. (A,B) Histological images of the complete heart sections (orcein) and a zoom of the A-V valve (A) or BA-V valve (B) from 12 months post fertilization fish stained with orcein (first row), immunolabeled with anti-elastin antibodies (second row), or stained with picrosirius red (third row). (C) Quantification of “honeycomb-like” structures referred to as alveolate areas in valves from histological sections of zebrafish hearts. Each point is the result of one leaflet of the valve. Circles are for A-V valves, and squares are for V-BA valves. Four fish per genotype were analyzed; however, all their valves were not always visible enough to perform the analysis. (D) Global aspect of each valve leaflet was categorized as normal or pathological when the shape was unusual and the alveolate area exceeded 20% of the total surface. Scale bar = 200µm for complete heart sections and 40µm for valve images; A = atrium; V = ventricle; BA = bulbus arteriosus; SV = sinus venosus; A-V = atrio-ventricular; V-BA = ventriculo-bulbar; WT = wild type (elna^+/+); HET = heterozygous (elna^sa12235/+); MUT = mutant (elna^{sa12235/sa12235}); Kruskall–Wallis test was performed followed by Dunnett’s post hoc test in (C); ns = not significant.