Fig. 7

Fig. 7. Blocking TGF-β signaling rescues bone dysplasia caused by Nox4 loss in early development. (A) Nine days postfertilization (dpf), WT, nox4−/−, and nox4−/− zebrafish treated with SB431542 were stained with alizarin red. The figures show the front view and an enlarged view of the tail. (B) Quantification of alizarin red staining in WT, nox4−/−, and nox4−/− zebrafish treated with SB431542 9 dpf. (C) At 8 dpf, Tg(sp7-EGFP) fluorescence in the control, GLX351322, and GLX351322 + SB431542 treatment groups was observed. (D) Quantification of Tg(sp7-EGFP) fluorescence in the control, GLX351322, and GLX351322 + SB431542 groups. (E) At 9 dpf, TRAP staining was performed on WT, nox4−/−, and nox4−/− zebrafish treated with SB431542. The upper panels show the lateral view and the lower panels show an enlarged view. (F) Quantification of TRAP staining. CB: ceratobranchial bone; PS: parasphenoid bone; OC: occipital bone; N: notochord; BR: branchial ray; OP: operculum; O: otolith. n = 10. The scale bars in panels A, C, E are 200 μm. The results were analyzed using one-way ANOVA. *P < 0.05, **P < 0.01, and ***P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)