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Fig. 6

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ZDB-FIG-230508-27

Publication

Cao et al., 2022 - Nox4 promotes osteoblast differentiation through TGF-beta signal pathway

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Fig. 6

Fig. 6. TGF-β signaling is dysregulated in nox4 −/− zebrafish. (A) WT and nox4 −/− transcriptomes were sequenced 9 days postfertilization (dpf) to identify differentially expressed genes. Up indicates upregulated genes and down indicates downregulated genes. (B) The differentially expressed genes were used for gene ontology (GO) analysis. The x-axis shows the enrichment fraction and the y-axis indicates the biological process. (C) The differentially expressed genes were used for Kyoto encyclopedia of genes and genes (KEGG) enrichment analysis. The x-axis shows the number of enriched genes in each pathway and the y-axis shows the signal pathway. (D, E) RT-qPCR analysis of Bmp, Tgf-β, and their receptor gene expression in WT and nox4−/− zebrafish. (F) Western blot of Smad2 and p-Smad2 protein expression downstream of TGF-β signaling pathway in WT and nox4−/− zebrafish. (G) Quantification of β-actin, Smad2, and p-Smad2 bands in WT and nox4−/− zebrafish. The results were analyzed using one-way ANOVA. *P < 0.05, **P < 0.01, and ***P < 0.001.

Expression Data

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Antibody Labeling

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Reprinted from Free radical biology & medicine, 193(Pt 2), Cao, Z., Liu, G., Zhang, H., Wang, M., Xu, Y., Nox4 promotes osteoblast differentiation through TGF-beta signal pathway, 595-609, Copyright (2022) with permission from Elsevier. Full text @ Free Radic. Biol. Med.