FIGURE

Fig. 7

ID

ZDB-FIG-230122-17

Publication

Gong et al., 2023 - Hyperaminoacidemia induces pancreatic α cell proliferation via synergism between the mTORC1 and CaSR-Gq signaling pathways

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Fig. 7

CaSR-Gq induces α cell proliferation via the ERK1/2 pathway.

a Representative images of pERK1/2 and GFP immunofluorescence in islet sections of control, gcgr^DKO, casr−/−;gcgr^DKO and Tgα^CaSR;casr−/−;gcgr^DKO larvae at 5 dpf. The pERK1/2 signal in α cells is indicated by arrows, primary antibody: Anti-pERK1/2 (Thr202/Tyr204) (1:150, rabbit); secondary antibody: Alexa Fluor 568 (1:1,000, goat anti-rabbit) (scale bar, scale bar, 8 μm). b Quantification of the percentage of pERK1/2 positive α cells in control, gcgr^DKO, casr−/−;gcgr^DKO and Tgα^CaSR;casr−/−;gcgr^DKO larvae at 5 dpf (data represent the means ± SD, n = 10 for each group). c Quantification of pERK1/2 fluorescence intensity in α cell of control, gcgr^DKO, casr−/−;gcgr^DKO, and Tgα^CaSR;casr−/−;gcgr^DKO larvae at 5 dpf (Data represent means ± SD, n = 9 for each group). d Representative images of pERK1/2 and GFP immunofluorescence in islet sections of control, Tgα^Gq;casr−/−;gcgr^DKO and Tgα^Gq;gcgr^DKO larvae at 5 dpf. The larvae were treated with 20 μM CNO or vehicle for 48 h. The pERK1/2 signal in α cells is indicated by arrows, primary antibody: Anti-pERK1/2 (Thr202/Tyr204) (1:150, rabbit); secondary antibody: Alexa Fluor 568 (1:1000, goat anti-rabbit) (scale bar, 8 μm). e Quantification of the percentage of pERK1/2 positive α cells in control, Tgα^Gq;casr−/−;gcgr^DKO and Tgα^Gq;gcgr^DKO larvae treated with 20 μM CNO or vehicle for 48 h at 5 dpf (data represent the means ± SD, n = 9 for each group). f Quantification of the pERK1/2 fluorescence intensity in α cells of control, Tgα^Gq;casr−/−;gcgr^DKO and Tgα^Gq;gcgr^DKO larvae treated with 20 μM CNO or vehicle for 48 h at 5 dpf (data represent the means ± SD, n = 10 for each group). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, NS indicates no significant difference (one-way ANOVA, Tukey’s multiple comparisons test, the quantifications represent individual islet sections). Source data are provided as a ^{Source Data} file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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