Fig. 6

a Representative images of EdU staining in control, Tgα^CA-Rheb, Tgα^Gq, and Tgα^CA-Rheb;Tgα^Gq larvae treated with 1 μM CNO or vehicle for 48 h at 5 dpf. EdU (red)-positive α cells are indicated by arrows (scale bar, 10 μm). b Quantification of α cell numbers in control, Tgα^CA-Rheb, Tgα^Gq, and Tgα^CA-Rheb;Tgα^Gq larvae treated with 1 μM CNO or vehicle for 48 h at 5 dpf. (data represent the means ± SD, n = 8, 8, 6, 8, 8, 6, 9, and 11 for each group, respectively). c Quantification of EdU-positive α cell numbers in control, Tgα^CA-Rheb, Tgα^Gq, and Tgα^CA-Rheb;Tgα^Gq larvae treated with 1 μM CNO or vehicle for 48 h at 5 dpf (data represent the means ± SD, n = 9 for each group). d Representative images of α cells in control, gcgr^DKO, and Tgα^Gq larvae treated with 20 μM CNO or vehicle for 48 h at 5 dpf (scale bar, 10 μm). e Quantification of α cell numbers in control, gcgr^DKO and Tgα^Gq larvae treated with 20 μM CNO or vehicle for 48 h at 5 dpf (data represent the means ± SD, n = 9, 9, 9, 9, 5, and 5 for each group, respectively). f Representative images of EdU staining in control, gcgr^DKO and Tgα^Gq larvae treated with 20 μM CNO or vehicle for 48 h at 5 dpf. EdU (red)-positive α cells are indicated by arrows (scale bar, 10 μm). g Quantification of EdU-positive α cell numbers in control, gcgr^DKO and Tgα^Gq larvae treated with 20 μM CNO or vehicle for 48 h at 5 dpf (data represent the means ± SD, n = 10, 10, 10, 10, 5, and 5 for each group, respectively). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, NS indicates no significant difference (one-way ANOVA, Tukey’s multiple comparisons test, the quantifications represent individual islet sections). Source data are provided as a ^{Source Data} file.