FIGURE

Fig. 6

ID

ZDB-FIG-230122-16

Publication

Gong et al., 2023 - Hyperaminoacidemia induces pancreatic α cell proliferation via synergism between the mTORC1 and CaSR-Gq signaling pathways

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Fig. 6

Synergism of the Gq and mTORC1 pathways is sufficient to induce ? cell proliferation.

a Representative images of EdU staining in control, Tg?^CA-Rheb, Tg?^Gq, and Tg?^CA-Rheb;Tg?^Gq larvae treated with 1 ?M CNO or vehicle for 48 h at 5 dpf. EdU (red)-positive ? cells are indicated by arrows (scale bar, 10 ?m). b Quantification of ? cell numbers in control, Tg?^CA-Rheb, Tg?^Gq, and Tg?^CA-Rheb;Tg?^Gq larvae treated with 1 ?M CNO or vehicle for 48 h at 5 dpf. (data represent the means � SD, n = 8, 8, 6, 8, 8, 6, 9, and 11 for each group, respectively). c Quantification of EdU-positive ? cell numbers in control, Tg?^CA-Rheb, Tg?^Gq, and Tg?^CA-Rheb;Tg?^Gq larvae treated with 1 ?M CNO or vehicle for 48 h at 5 dpf (data represent the means � SD, n = 9 for each group). d Representative images of ? cells in control, gcgr^DKO, and Tg?^Gq larvae treated with 20 ?M CNO or vehicle for 48 h at 5 dpf (scale bar, 10 ?m). e Quantification of ? cell numbers in control, gcgr^DKO and Tg?^Gq larvae treated with 20 ?M CNO or vehicle for 48 h at 5 dpf (data represent the means � SD, n = 9, 9, 9, 9, 5, and 5 for each group, respectively). f Representative images of EdU staining in control, gcgr^DKO and Tg?^Gq larvae treated with 20 ?M CNO or vehicle for 48 h at 5 dpf. EdU (red)-positive ? cells are indicated by arrows (scale bar, 10 ?m). g Quantification of EdU-positive ? cell numbers in control, gcgr^DKO and Tg?^Gq larvae treated with 20 ?M CNO or vehicle for 48 h at 5 dpf (data represent the means � SD, n = 10, 10, 10, 10, 5, and 5 for each group, respectively). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, NS indicates no significant difference (one-way ANOVA, Tukey?s multiple comparisons test, the quantifications represent individual islet sections). Source data are provided as a ^{Source Data} file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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