FIGURE

Fig. 2

ID

ZDB-FIG-230122-11

Publication

Gong et al., 2023 - Hyperaminoacidemia induces pancreatic α cell proliferation via synergism between the mTORC1 and CaSR-Gq signaling pathways

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Fig. 2

Activation of mTORC1 is insufficient for α cell proliferation.

a Representative images of α cells in control, Tgα^CA-MTOR (Tg(gcga:MTOR^L1460P, cryaa:tagRFP)), Tgα^CA-Rheb (Tg(gcga:Rheb^S16H, cryaa:tagYFP)), and gcgr^DKO (gcgra−/−;gcgra−/−) larvae at 5 dpf (scale bar, 10 μm). b Quantification of α cell number in control, Tgα^CA-MTOR, Tgα^CA-Rheb and gcgr^DKO larvae at 5 dpf (data represent the means ± SD, n = 15 for each group). c Quantification of α cell size in control, Tgα^CA-MTOR, Tgα^CA-Rheb and gcgr^DKO larvae at 5 dpf (Data represent means ± SD, n = 16 for each group). d Representative images of EdU staining in control, Tgα^CA-MTOR and Tgα^CA-Rheb larvae at 5 dpf. EdU (red)-positive α cells are indicated by arrows (scale bar, 10 μm). e Quantification of EdU-positive α cell numbers in control, Tgα^CA-MTOR and Tgα^CA-Rheb larvae at 5 dpf (data represent the means ± SD, n = 9 for each group). ***P < 0.001, ****P < 0.0001, NS indicates no significant difference (one-way ANOVA, Tukey’s multiple comparisons test, the quantifications represent individual islet sections). Source data are provided as a ^{Source Data} file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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