Fig. 2

a RNA-seq on WT and MZsmad4a embryos performed in triplicate. In the heatmap the color legend shows scaled RLog transformed expression counts for each replicate. b RNA-seq reads for representative BMP (upper panel) and Nodal (bottom panel) target genes in WT and MZsmad4a embryos. The data are means ± SD from three biological replicates for each genotype. sizzled: p(adj) = 4.699 × 10^-78, smad1: p(adj) = 9.253 × 10^-23, eve1: p(adj) = 1.581 × 10^-27, bmp4: p(adj) = 2.183 × 10^-23, id3: p(adj) = 2.445 × 10^-48. Wald test. ****p(adj) < 0.0001. c qPCR for ndr1/2 and lft1/2 in WT and MZsmad4a embryos at the indicated stages. Normalized values are shown as means ± SEM. For ndr1/2, the data are the result of three biological replicates while for lft1/2 they are the result of four biological replicates, except for lft1 in MZsmad4a embryos at sphere stage and lft2 in WT embryos at 30% epiboly, which are the result of three biological replicates. lft1 30% epiboly: p(adj) = 4389 × 10^-5, lft2 sphere: p(adj) = 0.046, lft2 30%: p(adj) = 0.002. Unpaired multiple comparison t-test with Holm-Sidack correction. *p < 0.05; **p < 0.01; ****p < 0.0001.