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Expression of neutrophil and PRC1 markers decreased with <italic>asxl1</italic> mutation.A Heat map of differentially expressed genes (false discovery rate < 0.05; exact test) in 3 dpf asxl1−/− compared to asxl1+/+ via RNA-seq. Neutrophil markers (green), PRC1 members (orange), and inflammatory response associated genes (purple) were highlighted. Rep replicate. B Gene ontology (GO) enrichment analysis of biological processes based on differentially expressed genes identified through RNA-seq (hypergeometric test). C Expression of bmi1a and cbx4 from RNA-seq (n = 20 per replicate, CPM count-per-million; exact test, *false discovery rate (FDR) < 0.05, **FDR < 0.01, ***FDR < 0.001; error bars, mean ± SD). qRT-PCR comparing expression of bmi1a and cbx4 in asxl1+/+ and asxl1−/− in 3 dpf larvae (n ≥ 10 per group, performed with four replicates; two-tailed Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001; error bars, mean ± SD) (color figure online).
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