FIGURE

Fig. 2

ID

ZDB-FIG-210504-17

Publication

Peng et al., 2020 - Induction of Wnt signaling antagonists and p21-activated kinase enhances cardiomyocyte proliferation during zebrafish heart regeneration

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Fig. 2

Suppression of Wnt signaling enhances heart regeneration by increasing CM proliferation. (A) Schematic of heat shock experiments and Wnt agonist treatment for CM proliferation, qPCR, and AFOG analyses. Heat shock (HS): 37�C for 2 h for control and Tg(hsp70:dkk1b) animals at 4, 5, and 6 dpa for PCNA/Mef2C assay or over the time period from 4 to 21 dpa for AFOG analysis; 37�C for 4 h for control and Tg(hsp70:wnt8a) animals at 6 dpa for PCNA/Mef2C assay or over the time period from 6 to 30 dpa for AFOG analysis. EdU: injection at 5 and 6 dpa. CHIR99021: 20 �M (in DMSO) from 6 to 7 dpa. Control: 0.1% DMSO from 6 to 7 dpa. (B?I) Confocal microscopy analyses for CM proliferation. Insets show high-magnification images in rectangles. Scale bar, 100 �m. Data are mean � SEM from five hearts each. Student?s t-test, *P?

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Mol. Cell Biol.