FIGURE

Fig. 1

ID

ZDB-FIG-201117-8

Publication

Xia et al., 2020 - Slc39a5-mediated zinc homeostasis plays an essential role in venous angiogenesis in zebrafish

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Fig. 1

slc39a5 morphant zebrafish embryos develop cardiac ischaemia and blood cell accumulation in the caudal vein plexus. (a) Quantitative PCR analysis of the 12 indicated slc39a genes measured in wild-type (WT) embryos and embryos treated with 1 mM zinc (+Zn). Note zebrafish lacking slc39a2 and slc39a12. (b) In situ hybridization of WT zebrafish embryos using the antisense and sense (control) slc39a5 probes. Note the concentrated expression in the head and gut region at 3 dpf (red arrow). (c–d) Representative images (c) and o-Dianisidine-stained (d) control and scl39a5 morphant embryos at the indicated developmental stages. The red arrows indicate the heart region. (e) Representative images of GFP fluorescence measured in the caudal region of control and slc39a5 morphant Tg(globinLCR:eGFP) embryos. Note the accumulation of RBCs in the CVP of slc39a5 morphants. (f) Whole-mount in situ hybridization of the RBC marker hbea3 in control and slc39a5 morphants. Note the increased signal in the CVP of the slc39a5 morphant (arrow).

Expression Data

Genes:	hbae3 slc39a5 slc39a7 slc39a10 slc39a11
Fish:	WT cz3325Tg + MO1-slc39a5
Condition:	chemical treatment by environment: zinc atom
Knockdown Reagent:	MO1-slc39a5
Anatomical Terms:	caudal vein plexus gut head whole organism
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-slc39a5 cz3325Tg + MO1-slc39a5
Condition:	chemical treatment by environment: zinc atom
Knockdown Reagent:	MO1-slc39a5
Observed In:	caudal vein plexus caudal vein plexus nucleate erythrocyte heart blood circulation whole organism
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

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