Figure 5.

(A) After Aβ^short injection into WT larvae (top right), c-fos is detected in many larval brain areas, including the dorsal and ventral telencephalon and posterior hypothalamus (black arrowheads), but not after injection of Aβ reverse controls (left). In contrast, Aβ^short injections into either adrb2a^-/- (middle right) or pgrmc1^-/- mutants (bottom right) do not induce c-fos expression. The brains in the middle and lower panels were stained longer than the WT (+/+) brains to ensure detection of weaker expression. D = dorsal, p=Posterior. n = blinded counts of brains with expression pattern/total brains. (B) Compared to Aβ^rev injections, Aβ^long oligomers induce less c-fos expression in WT larvae (top panels). In contrast, Aβ^long induced relatively increased c-fos expression in the telencephalon and posterior hypothalamus (black arrows) in the prp1^-/-; prp2 ^-/- double mutants. These Aβ^rev and Aβ^longinjectedbrains were stained longer to ensure detection of weaker c-fos expression. D = dorsal, p=Posterior.