Figure 1—figure supplement 3.

(A–B) The effects of Aβ^short (A) and Aβ^long (B) on waking activity (top) and sleep (bottom) return to baseline after 24 hr. Shown in A and B are traces for 48 hr post-injection on a 14 hr:10 hr light:dark cycle (indicated by the white and black bars). The ribbons represent ±the SEM. Plotted on the right are the percent change induced by Aβ relative to Aβ^rev for day 1 versus day 2, demonstrating that these parameters return to baseline (*p≤0.01, paired t-test). Each line represents a single larva, the thicker line represents the mean ± SEM. (C) Activity traces of Aβ^short (blue), Aβ^long (green), and Aβ^rev (black) during and after a ten minute dark pulse are indistinguishable. Traces are for a 10 min:10 min:10 min light:dark:light window (indicated by the white and black bars). The ribbons represent ±the SEM. (D) The effects of Aβ^rev (black), convulsant PTZ (10 mM, orange), Aβ^short (blue), and Aβ^long (green) on sub-second larval bout structures. Shown are delta pixel movement of one representative larva in each group for ~1 min (see Figure 1—video 1). Unlike PTZ treated larvae, Aβ injected larvae have normal bout structures. Representative larvae were chosen to also highlight that, while overall Aβ^long injected larvae are less active, individual animals can have sustained periods of heightened activity. Similarly, individual Aβ^short injected animals can exhibit periods of relatively dampened activity. (E) The effects of Aβ^rev (black), convulsant PTZ (10 mM, orange), Aβ^short (blue) and Aβ^long (green) on high-frequency bouts (HFB) in a 5 min interval. Each dot represents a single larva. PTZ-induced HFB’s is non-existent or very rare in the other groups (n = 24 for all groups).