FIGURE

Figure 3—figure supplement 1.

ID

ZDB-FIG-200718-50

Publication

Özcan et al., 2020 - Sleep is bi-directionally modified by amyloid beta oligomers

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Figure 3—figure supplement 1.

Crispr/Cas9 targeting of zebrafish <italic>adrb2a</italic> and <italic>pgrmc1.</italic>

(A) Human PGRMC1and zebrafish Pgrmc1 contain a conserved Transmembrane (TM) domain (yellow) and a Cytochrome b5-like Heme/Steroid binding domain (blue). (B) Protein alignment of zebrafish Pgrmc1 to human PGRMC1. Identical residues are marked in black, similar residues in grey. (C) Crispr-Cas9 targeting of pgrmc1 generated an allele with a 16 bp deletion. The PAM sequence is indicated in red. (D) The predicted translation of pgrmc1 Δ16 leads to a premature stop codon. (E) Protein alignment of zebrafish Adrb2a and Adrb2b to human ADRB2. Identical residues are marked in black, similar residues in grey. The N-terminal region (pink) is the putative Aβ binding region. The C-terminal region is in green. (F) Crispr-Cas9 targeting of adrb2a generated an allele with an 8 bp deletion. The PAM sequence is indicated in red. (G) The predicted translation of adrb2aΔ8 leads to a premature stop codon lacking all functional domains.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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