FIGURE

Fig. 1

ID

ZDB-FIG-200306-49

Publication

Lee et al., 2020 - REEP5 depletion causes sarco-endoplasmic reticulum vacuolization and cardiac functional defects

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

REEP5 is an evolutionarily conserved, muscle-enriched membrane protein.

a A multispecies alignment of REEP5 from vertebrates. b Prediction of human REEP5 protein topography generated by TOPCONS. c Membrane yeast two-hybrid assay of REEP5 membrane topology. SD-WL is yeast media that lacks tryptophan and leucine and selects for cells that contain both bait and prey plasmids. SD-WLAH +10 nM 3-AT is yeast media that lacks tryptophan, leucine, adenine, and histidine and selects for cells in which bait and prey are interacting. d Predicted membrane topology model of human REEP5 generated by modification of a T(E)Xtopo output in Protter (http://wlab.ethz.ch/protter). e REEP5 mRNA transcript levels obtained from Human Protein Atlas across various mouse tissues. f Immunoblot of REEP5 protein expression in mouse tissues. Asterisks to the right indicate the number of predicted REEP5 oligomers detected based on anticipated molecular weight. g Immunodepletion of REEP5 antigens with bacterially expressed 6xHis-REEP5. h GEO RNA-seq datasets demonstrate changes in REEP5 expression across various mouse and human cardiovascular diseases; * indicates a statistically significant p < 0.05 in a Tukey’s multiple comparison analysis. Data are presented as mean ± SEM with n = 3 biologically independent measurements. i Immunoblot analysis of REEP5 and BNP expression from cardiac tissue of hypertrophic cardiomyopathy (HCM), myocardial infarction (MI), and dilated cardiomyopathy (DCM) mouse models. j Immunoblot analysis of REEP5, BNP, and MHC expression from human cardiac samples of normal, idiopathic, and ischemic cardiomyopathy. Source data containing original uncropped immunoblots are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.