Fig. 1

Fig. 1

a A multispecies alignment of REEP5 from vertebrates. b Prediction of human REEP5 protein topography generated by TOPCONS. c Membrane yeast two-hybrid assay of REEP5 membrane topology. SD-WL is yeast media that lacks tryptophan and leucine and selects for cells that contain both bait and prey plasmids. SD-WLAH +10 nM 3-AT is yeast media that lacks tryptophan, leucine, adenine, and histidine and selects for cells in which bait and prey are interacting. d Predicted membrane topology model of human REEP5 generated by modification of a T(E)Xtopo output in Protter (http://wlab.ethz.ch/protter). e REEP5 mRNA transcript levels obtained from Human Protein Atlas across various mouse tissues. f Immunoblot of REEP5 protein expression in mouse tissues. Asterisks to the right indicate the number of predicted REEP5 oligomers detected based on anticipated molecular weight. g Immunodepletion of REEP5 antigens with bacterially expressed 6xHis-REEP5. h GEO RNA-seq datasets demonstrate changes in REEP5 expression across various mouse and human cardiovascular diseases; * indicates a statistically significant p < 0.05 in a Tukey’s multiple comparison analysis. Data are presented as mean ± SEM with n = 3 biologically independent measurements. i Immunoblot analysis of REEP5 and BNP expression from cardiac tissue of hypertrophic cardiomyopathy (HCM), myocardial infarction (MI), and dilated cardiomyopathy (DCM) mouse models. j Immunoblot analysis of REEP5, BNP, and MHC expression from human cardiac samples of normal, idiopathic, and ischemic cardiomyopathy. Source data containing original uncropped immunoblots are provided as a Source Data file.